GENETICS, MOLECULAR BIOLOGY

Over the last three decades, knowledge on the molecular biology of human cancers has vastly expanded. A host of genes and proteins involved in cancer development and progression have been defined and many mechanisms at the molecular, cellular and even tissue level have been, at least partly, elucidated. Insights have also been gained into the molecular mechanisms underlying carcinogenesis by chemical, physical, and biological agents and into inherited susceptibility to cancer.

Accordingly, Part I of the book presents many of the molecules and mechanisms generally important in human cancers. Following an overview on the cancer problem, individual chapters deal with cancer genetics and epigenetics, DNA damage and repair, oncogenes, tumor suppressors, regulatory pathways in cancer, apoptosis, cellular senescence, tumor invasion, and metastasis.

A consensus is emerging that while these common mechanisms and molecules are all relevant to human cancers, in each cancer type (or even subtype) a selection of them are extremely important. For selected cancers, the route from genetic and epigenetic changes to their biological and clinical behavior can already be traced. Part II of the book presents a broad, but exemplary selection of cancers that serve as paradigms to illustrate this point.

In fact, cancer research has now reached a critical stage, in which the accumulated knowledge on molecular mechanisms is gradually translated into improved prevention, diagnosis, and treatment. The state, pitfalls, and potential of these efforts are summarized in Part III.

More than ever, cancer research is now an interdisciplinary effort which requires a basic knowledge of commonly used terms, facts, issues, and concepts. The aim of this book is to provide advanced students and practitioners of different disciplines with this basis, bridging the gap between standard textbooks of molecular biology, pathology, and
oncology on the one hand and the specialized cancer literature on the other.

Code:

http://www.megaupload.com/?d=26E3EBSH ________________________________________________

The Double Helix : A Personal Account of the Discovery of the DNA

ISBN: 074321630X Title: The Double Helix : A Personal Account of the Discovery of the Structure of DNA Author: James D. Watson Publisher: Touchstone Quote: Richard Feynman He has described admirably how it feels to have that frightening and beautiful experience of making a scientific discovery. Book Description By identifying the structure of DNA, the molecule of life, Francis Crick and James Watson revolutionized biochemistry and won themselves a Nobel Prize. At the time, Watson was only twenty-four, a young scientist hungry to make his mark. His uncompromisingly honest account of the heady days of their thrilling sprint against other world-class researchers to solve one of science's greatest mysteries gives a dazzlingly clear picture of a world of brilliant scientists with great gifts, very human ambitions, and bitter rivalries. With humility unspoiled by false modesty, Watson relates his and Crick's desperate efforts to beat Linus Pauling to the Holy Grail of life sciences, the identification of the basic building block of life. Never has a scientist been so truthful in capturing in words the flavor of his work pass: ebooksclub.org Code: http://www.bigfileupload.com/Smiley_for_ebooksclub/074321630X/

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Two great Molecular Biology Book at one place
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The Rar files contain downloaded web pages fro www.ncbi.nlm.nih.gov
The files were leeched by Internet researcher Version 2.1
As other software like offline explorer does not support leeching javascript protected webpages, this software does. I did not export the pages due to javascript problems. So best way is to read the project file (Whole book) with all links in the programs internal browser. I have included the program with this rar file.

Human Molecular Genetics 2
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ISBN 0-471-33061-2
Tom Strachan & Andrew P. Read
Second edition, BIOS Scientific Publishers Ltd, 1999
Info: http://www.wiley.com/legacy/products/subject/life/genetics/strachan_index.html

Genomes
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ISBN 0-471-25046-5
T. A. BROWN
Second Edition, BIOS Scientific Publishers Ltd, 2002
Info: http://www.wiley.com/legacy/products/subject/life/genetics/brown_index.html

Others may follow the example to download some more biology (especially microbiology) books and post the links on this forum. Due to my slow connection I am uploading the file (total 24 MB in 5 parts). Sorry for that.


Links:
Part1
http://rapidshare.de/files/8603703/Genomes__Molecular_Human_Genetics.part1.rar.html

Part2
http://rapidshare.de/files/8606069/Genomes__Molecular_Human_Genetics.part2.rar.html

Part3
http://rapidshare.de/files/8608460/Genomes__Molecular_Human_Genetics.part3.rar.html

Part4
http://rapidshare.de/files/8619094/Genomes__Molecular_Human_Genetics.part4.rar.html

Part5
http://rapidshare.de/files/8619270/Genomes__Molecular_Human_Genetics.part5.rar.html

Password: moon_far_side

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Series: Methods in Molecular Biology, Methods and Protocols

A/poptosis Meth/ods and Proto/cols
Bra/dy, Hu/gh J. M. (University College London, UK)
Abstract:
A collection of cutting-edge techniques for detecting and quantifying apoptosis, understanding its biochemistry, and for identifying the genes and proteins that regulate and carry it out. Described in step-by-step detail, these readily reproducible methods range from flow cytometry and immunohistochemical procedures to kinase activity assays, yeast two-hybrid screening, and the cloning of novel genes by differential expression. The protocols follow the successful Methods in Molecular Biology™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Apoptosis Methods and Protocols constitutes a key technical reference to the significant methodologies used in the field, as well as offering novice and experienced researchers alike powerful tools to illuminate the phenomenon of programmed cell death.
Reviews:
“The calibre of the contributors to this book, make it an authoritative tome. As it also provides insights in to how and why specific protocols should be applied, it will be a great asset to those studying apoptosis in laboratory-based haematology.” –British Journal of Haematology

Series: Methods in Molecular Biology
Volume #: 282
Binding: Hardcover

code:

hXXp://rapidshare.de/files/1026471/Book1.rar.html
password: CuttingEdgeTechniques

Apoptosis Methods and Protocols
hXXp://rapidshare.de/files/1026534/Book2.rar.html
password: ExperiencedResearchers

Pages: 320
ISBN: 0-89603-873-4


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Genetics (Macmillan Science Library) (Vol. 1-4) Code: http://rapidshare.de/files/3932884/R.Robinson_et_al_-_Macmillan_Encyclopedia_of_Genetics._Vol.1-4.rar.html Password: www.AvaxHome.ru Richard Robinson (Editor), "Genetics (Macmillan Science Library)" (Vol. 1-4) MacMillan Reference Books | ISBN 0028656067 | 2003 Year | DjVu | 24 Mb | 308/259/267/306 Pages This encyclopedia set, part of the Macmillan Science Library series, is intended for use by middle- and high-school students, college nonspecialists, and beginning researchers. More than 100 contributors are responsible for approximately 250 signed entries from Accelerated aging: Progeria to Zebrafish. Articles range from a few paragraphs to a few pages in length and focus on a variety of topics, including inheritance, genes and chromosomes, genetic diseases, biotechnology, history, careers, and the ethical, legal, and social issues associated with genetically modified foods and cloning. The entries appear in alphabetical order and include cross-references to related entries. Most have a list of suggested readings and Internet resources to examine for further information. Each volume begins with a complete table of contents as well as a "For Your Reference" section that includes a group of diagrams and illustrations to help students visualize the various structures related to DNA and RNA. Words that appear in bold in the text are defined in the page margins and collected in a glossary at the conclusion of each volume. Each volume also includes a topical outline and an index, and volume 4 includes a cumulate index as well. The attractive layout features a wealth of colorful diagrams and photographs that will appeal to students as well as the casual browser. The clear and well-written articles are informative and should meet the needs of most students. In addition, users will appreciate the thorough indexing and cross-referencing, which make navigation straightforward. Though it has more extensive coverage, World of Genetics [RBB Je 1 & 15 02] lacks suggested readings at the end of each entry and is not as attractively designed. It is aimed at the high-school level and up, while Encyclopedia of Genet ics (Salem, 1999) is designed for use by undergraduates and others with a general understanding of science. Genetics would be a useful resource for middle- and high-school libraries as well as undergraduate and public libraries.

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Analysis of Genes and Genomes

Richard J. Reece, «Analysis of Genes and Genomes» | John Wiley & Sons | 2004 Year | PDF | 7,38 Mb | 491 Pages



“Reece (University of Manchester, UK) introduces the theoretical and practical basis of genetic engineering, gene cloning, and molecular biology, using an example-driven approach to present fundamentals of creating mutations in DNA and cloning in bacteria, yeast, plants, and animals. All aspects of genetic engineering in the post-genomic era are covered, beginning with the basics of DNA structure and DNA metabolism, with new technologies, such as DNA macroarrays, are introduced in later chapters. Key concepts, color explanatory diagrams, and a list of acronyms are included. The text is aimed at students with a basic knowledge of the molecular side of biology”.

Code:
http://rapidshare.de/files/1482593/John.Wiley.and.Sons.Analysis.of.Genes.and.Genomes.eBook-YYePG.zip.html

From the contents
Preface.

Acknowledgements.

Dedication.

1. DNA: structure and function.

1.1 Nucleic acid is the material of heredity.

1.2 Structure of nucleic acids.

1.3 The double helix.

1.4 Reversible denaturing of DNA.

1.5 Structure of DNA in the cell.

1.6 The eukaryotic nucleosome.

1.7 The replication of DNA.

1.8 DNA polymerases.

1.9 The replication process.

1.10 Recombination.

1.11 Genes and genomes.

1.12 Genes within a genome.

1.13 Transcription.

1.14 RNA processing.

1.15 Translation.

2. Basic techniques in gene analysis.

2.1 Restriction enzymes.

2.2 Joining DNA molecules.

2.3 The basics of cloning.


2.4 Bacterial transformation.

2.5 Gel electrophoresis.

2.6 Nucleic acid blotting.

2.7 DNA purification.

3. Vectors.

3.1 Plasmids.


3.2 Selectable markers.

3.3 l Vectors.

3.4 Cosmid vectors.

3.5 M1 3 vectors.

3.6 Phagemids.

3.7 Artificial chromosomes.

4. Polymerase chain reaction.

4.1 PCR reaction conditions.

4.2 Thermostable DNA polymerases.

4.3 Template DNA.

4.4 Oligonucleotide primers.

4.5 Primer mismatches.

4.6 PCR in the diagnosis of genetic disease.

4.7 Cloning PCR products.

4.8 RT-PCR.

4.9 Real-time PCR.

4.10 Applications of PCR.

5. Cloning a gene.

5.1 Genomic libraries.


5.2 cDNA libraries.

5.3 Directional cDNA cloning.

5.4 PCR-based libraries.

5.5 Subtraction libraries.

5.6 Library construction in the post-genome era.

6. Gene identification.

6.1 Screening by nucleic acid hybridization.

6.2 Immunoscreening.

6.3 Screening by function.

6.4 Screening by interaction.

6.5 Phage display.

6.6 Two-hybrid screening.

6.7 Other interaction screens - variations on a theme.

7. Creating mutations.

7.1 Creating specific mutations.

7.2 Primer extension mutagenesis.

7.3 Strand selection methods.

7.4 Cassette mutagenesis.

7.5 PCR-based mutagenesis.

7.6 QuikChange(r) mutagenesis.

7.7 Creating random mutations in specific genes.

7.8 Protein engineering.

8. Protein production and purification.

8.1 Expression in E. coli.

8.2 Expression in yeast.

8.3 Expression in insect cells.


8.4 Expression in higher eukaryotic cells.

8.5 Protein purification.

9. Genome sequencing projects.

9.1 Genomic mapping.

9.2 Genetic mapping.

9.3 Physical mapping.

9.4 Nucleotide sequencing.

9.5 Genome sequencing.

9.6 The Human Genome Project.

9.7 Finding genes.

9.8 Gene assignment.

9.9 Bioinformatics.

10. Post-genome analysis.

10.1 Global changes in gene expression.

10.2 Protein function on a genome-wide scale.

10.3 Knock-out analysis.

10.4 Antisense and RNA interference (RNAi).

10.5 Genome-wide two-hybrid screens.

10.6 Protein-detection arrays.

10.7 Structural genomics.

11. Engineering plants.

11.1 Cloning in plants.

11.2 Commercial exploitation of plant transgenics.

11.3 Ethics of genetically engineered crops.

12. Engineering animal cells.

12.1 Cell culture.

12.2 Transfection of animal cells.

12.3 Viruses as vectors.

12.4 Selectable markers and gene amplification in animal cells.

12.5 Expressing genes in animal cells.

13. Engineering animals.

13.1 Pronuclear injection.

13.2 Embryonic stem cells.

13.3 Nuclear transfer.

13.4 Gene therapy.

13.5 Examples and potential of gene therapy.

Glossary.

Appendices.

Nobel prize winners.

References.

Index.